The first slide shows a diagramatic view of the column with many small silica particles (in green).Ģ. Reversed-phase chromatography is all about the particle surface of the HPLC/UPLC column and the eluent solvent. Sorry, either Adobe flash is not installed or you do not have it enabled Transcript:ġ. To rewind the animation move the scroll bar to the left. Press the play button in order to move ahead in the animation. The animation below will explain the principle of reversed-phase chromatography. Animation of Reversed-Phase Chromatography (Principle) Today reverse phase chromatography surfaces are used with HPLC (high pressure liquid chromatography) and even UPLC (ultra pressure liquid chromatography) systems making it a very useful tool for analytical chemists. ( Note- check the animation for the principle of thin layer chromatography to understand how normal phase works) The primary difference between reversed-phase chromatography and normal phase chromatography is that the stationary phase in RPC utilizes a non-polar or hydrophobic surface as opposed to a polar (Si-OH) surface used in normal phase chromatography. Reversed-phase chromatography (abbreviated as RPC) is an important analytical technique which is widely used by analytical chemists in pharmaceuticals, forensics, food technology, biotechnology and even medicine.
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